Jan. 15, 2022
When the laser spectrograph analyzes the sample, the sample does not need to be processed in advance, nor does it destroy the oiginal shape of the sample. The laser spectrograph has simple operation and fast analysis speed. It is widely used in production and scientific research fields such as geology, metallurgy, medicine, archaeology, biology and so on.
In the analysis of biological samples, the laser spectrometer includes Laser Raman Spectroscopy, Laser Scattering Spectroscopy, Laser-induced Fluorescence Spectroscopy, Laser Photoacoustic Spectroscopy, Laser dissociation time-of-flight Mass Spectrometry, and so on.
The laser spectrum measurement is composed of four parts, including laser light source, vacuum system, optical system, analysis and detection system.
The light source system is a laser device for spectrum analysis. It consists of a laser, a laser power supply, a microscope, and a CCD monitoring system. The light emitted from the laser is focused by a objective lens of microscope to focus the beam on the sample. The surface of the sample absorbs laser energy and produces a strong photothermal effect. It melts and volatilizes the substance, forming a laser plasma, and then passes through a photoelectric receiving device for spectral analysis.
The laser beam enters the vacuum chamber through the imaging lens and the quartz window, and then focuses vertically on the surface of sample.
The optical system is composed of two parts. One is the transmission system of the laser, which consists of a optical reflector, a focusing objective lens, and a protective lens, which respectively function as light redirection, focusing, and lens protection to ensure that the laser beam is focused on the surface of sample. The other part is the illumination system with single-lens, whose function is to introduce the laser-induced plasma into the entrance slit of the spectrometer as much as possible.
Analysis and detection systems are usually divided into two ways. One is a spectroscopy method using a prism spectrometer as a spectroscopic system. Its working principle is that plasma induced by the laser is focused on the incident slit of the prism spectrometer through a lens. After the quartz prism splits, the spectrum information is obtained by processing. The other is a grating spectrometer.
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